5.1 Cylinder, 100-mL, graduated from 5 to 100 mL in 1.0-mL divisions, made of glass, heat-resistant glass, like borosilicate glass, or a chemical equivalent. The inside diameter shall be no less than 27 mm and no more than 30 mm throughout its length, measured from the top to a point 6 mm from the bottom of the cylinder. The overall height of the cylinder shall be 225 to 260 mm. The graduation shall not be in error by more than 1 mL at any point on the scale.
5.2 Heating Bath, sufficiently large and deep to permit the immersion of at least two test cylinders in the bath liquid up to their 85-mL graduations. The bath shall be capable of being maintained at a test temperature to within 61°C. The cylinder shall be secured in a position so that the longitudinal axis of the paddle corresponds to the vertical center line of the cylinder during the stirring operation. It is recommended that the bath be constructed with at least one transparent side that allows for clear visual inspection of the oil (fluid), water, and emulsion layer volumes while the cylinder remains immersed in the bath.
5.3 Stirring Paddle, made of chromium-plated or stainless steel and conforming to the following dimensions:
It is mounted on a vertical shaft of similar metal, approximately 6 mm in diameter, connected to a drive mechanism which rotates the paddle on its longitudinal axis at 1500 more or less 15 rpm. The apparatus is of such design that, when the cylinder is clamped in position and the paddle assembly is lowered into the cylinder, a positive stop engages and holds the assembly when the lower edge of the paddle is 6 mm from the bottom of the cylinder. During the operation of the stirrer, the center of the bottom edge of the paddle shall not deviate more than 1 mm from the axis of rotation. When not in operation, the paddle assembly can be lifted vertically to clear the top of the graduated cylinder. (Warning - Paddle edges may be very sharp. Handle with care.) (Warning - A protective shield may be used to cover the rotating shaft of the stirrer.)
5.4 Glass Rod (Policeman), covered with a material such as rubber, resistant to the oil or fluid.
6. Reagents
6.1 Purity of Reagents - Reagent grade chemicals shall be used in all tests. Unless otherwise indicated, it is intended that all reagents shall conform to the specifications of the Committee on Analytical Reagents of the American Chemical Society, where such specifications are available.4 Other grades may be used, provided it is first ascertained that the reagent is of sufficiently high purity to permit its use without lessening the accuracy of the determination.
6.2 Reagent Water - Unless otherwise indicated, reference to water shall be understood to mean distilled, deionized water as defined by Type I or Type II in Specification D 1193 or Grade 3 in BS EN ISO 3696:1995.
6.3 Acetone, (Warning - Health hazard, Flammable.)
6.4 Toluene, (Warning - Flammable.) Solvents with similar cleaning and solvency characteristics may be substituted for toluene.
6.5 Detergent, free rinsing, anionic detergent with a pH of 9.5 to 11.