1. Some sealing gaskets needs to be heating for 1 - 2 hours at 300 degree Celsius before installtion;
2. Usually, the replacement is carried out at the room temperature. Do not secure too tight. When the temperature rises, the sealing gaskets will be tight naturally. The sample injection will be difficult if the sealing gasket is secured too tight, which often leads to the bending of syringe needle;
3. Usually, when replacing, it is better to take the column away from the injection chamber. After replacement, install the column accordingly.